Mark A. Ragan's A Biochemical Phylogeny of the Protists PDF

By Mark A. Ragan

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Extra resources for A Biochemical Phylogeny of the Protists

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Synechococcus spp. ) Chloroplasts of Acetabularia mediterranea of Chlamydomonas reinhardi of Chlorella ellipsoidea Reference 32 4 Nucleic Acids TABLE 1 (Continued) Organism of of of of of of of Chlorclla protothecaides Chlorclla pyrenoidosa Euglena gracilis strain Z E. gracilis var. ) Storck and Alexopoulos, 40-62 1970 except Sapromyccs sp. 27 Storck and Alexopoulos, 1970 Chytridiomycetes Mandel, 1968 Blastocladiclla emcrsonii 66 Mandel, 1968 44 Rhizophlyctis rosca Mandel, 1968 50 Rhizophydium sp.

S o m e of t h i s h y b r i d i z a t i o n m a y b e d u e t o t h e p r e s e n c e of s t r o n g l y c o n s e r v e d s e q u e n c e s , s u c h a s t h o s e c o d i n g for r R N A ( G r o o t et ai, for DNA-DNA DNA 1975). I n g e n e r a l , at least 7 5 % base pairing is r e q u i r e d a m b i e n t t e m p e r a t u r e s of Tm = 2 5 ° C ( D e L e y a n d K e r s t e r s , 1975). P a r t i c u - hybridization at larly t e n u o u s h y b r i d s c a n b e identified by t h e r m a l r e n a t u r a t i o n s t u d i e s o n t h e h e t e r o d u p l e x .

Coli, but the t R N A s p e c i e s for v a l i n e , m e t h i o n i n e , p h e n y l a l a n i n e , t r y p t o p h a n , t y r o s i n e , a l a n i n e , a n d i s o l e u c i n e a r e m o r e s i m i l a r t o e a c h o t h e r t h a n c o u l d be e x p e c t e d by c h a n c e . I n E. coli, s e q u e n c e s for t h r e o n y l - , g l y c y l - , i s o l e u c y l - , a n d t w o valylt R N A ' s e x h i b i t m o r e t h a n 6 6 % h o m o l o g y a m o n g t h e m s e l v e s ( C e d e r g r e n et ai, 1972; D a y h o f f , 1972a; C l a r k e a n d C a r b o n , 1974).

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