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The phenanthroline moiety remained c o v a l e n t l y attached t o the p r o t e i n , w h i l e the lanthanide complex broke down and luminescence was l o s t . Obviously, the thermodynamic s t a b i l i t y of these complexes, however high, was not sufficient to prevent their d i s s o c i a t i o n i n a very d i l u t e , buffered aqueous s o l u t i o n . These f i r s t unsuccessful attempts emphasized an important g e n e r a l i z a t i o n , namely, t h a t the time frame of l i g a n d exchange and metal exchange k i n e t i c s becomes a major c o n s i d e r a t i o n f o r complexes t o be used as probes i n b i o l o g i c a l systems.
Represent the coefficients and A, B , . . F represent flow cytometric parameters such as green fluorescence, red fluorescence, light scatter, etc. In a biplot (9,70) the principal component parameters are replaced by "row markers" which are proportional to principal components. g. green fluorescence). The length of each arrow is proportional to the standard deviation of the data represented by that parameter. The cosine of the angle between any two arrows is proportional to the correlation between the parameters (see Figure 5).
Biometrika 1971, 58: 453-467. 11. L. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1991. N. (North Holland), 1984, pp 23-30. 12. , Van der Ploeg, M. Science 1985, 230:1401-1403. 13. , In Cold Spring Harbor Symposia on Quantitative Biology, 1986 vol. 51, pp. 263-273. 14. M. J. Mol. Biol. 1975, 98: 503-517. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1991. ch003 R. C. Leif and L. M. , 690 West 20th Street, Hialeah, FL 33010 Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284 2 The synthesis of a novel series of functionalized macrocyclic complexes of the lanthanide (III) ions is reported.